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tary to the template and has a free 3 end onto which the growing strand can be elongated DNA polymerase can t prime itself it must have a 3 end to get started These primers are actually RNA A special RNA polymerase (primase) puts them in Later the RNA primer is removed (by RNAse H in eukaryotes and DNA polymerase I in prokaryotes), and the gaps are filled in by DNA polymerase This may be a mechanism to enhance the fidelity of DNA replication DNA replication proceeds in both directions from the replication origin (bidirectional), which means you need to form two sets of replication complexes Each replication complex moves away from the origin (in opposite directions), unwinding and replicating both strands at each replication fork The strands of DNA are wound around each other like the strands of a rope As the strands are pulled apart during the movement of a replication fork, this unwinding tends to make the ends of the DNA turn (imagine unwinding the strands of a rope) Since the DNA is very long, twisted, and wrapped around histones, the DNA really can t turn its ends are rather tied down The unwinding of one region around the replication forks introduces strain into the regions of DNA that are still double-stranded, tending to make them wind tighter This is called supercoiling of the DNA Proteins (topoisomerases) are present to relieve the strain associated with helix unwinding by nicking and rejoining the DNA in the double-stranded regions At each replication fork there are two DNA polymerase complexes As the double-stranded DNA is unwound, two template strands are exposed One of the templates can be replicated in a continuous fashion by DNA polymerase since a continuous synthesis of new strands can occur in the 5 to 3 direction as the template strand is exposed Since all growing chains must be synthesized in the 5 to 3 direction, the lagging chain must be continuously reinitiated as new template is exposed The lagging strand is then synthesized discontinuously, in pieces that must be joined together later After synthesis, the RNA primers must be removed, gaps filled in, and the strands joined to give a linear, duplex DNA New histones are added to the lagging strand (which is now a duplex) while the old histones remain with the leading strand As the smoke slowly clears, we have a copy of the original DNA Since all DNA polymerases require a primer and work only in the 5 to 3 direction, there s a problem with replicating the 5 ends of the DNA If an RNA primer has to be laid down and later removed, these ends can t get replicated For bacteria with a circular genome, this isn t a problem Eukaryotes have specialized structures called telomeres at the. c# .net pdf viewer Add a PDF viewer to a WPF application - Stack Overflow
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asp.net web api 2 for mvc developers pdf 4 Sep 2017 ... If I want to display a PDF file in the browser instead of downloading a copy, ... as byte - array , reading the content from a database, for example. asp.net pdf viewer user control ends of the chromosome to solve this problem The exact details aren t known, but telomeres at the ends of each chromosome consist of a larger number (3000 to 12,000 base pairs) of a tandem (side by side) repeat of a G-rich sequence, (TTAGGG)n, in human DNA A specialized enzyme, telomerase, that also contains an RNA cofactor is responsible for replication of at least one strand of these telomeric sequences use Thread::Signal; 15: You also need to create an event handler for the Page Load event, so the page will open with the user s up-to-date profile information. Create that event the same way you did earlier, and then add the body of the method, highlighted in Example 9-6. c# display pdf in winform how to read pdf file through C# ? - MSDN - Microsoft
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