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tary to the template and has a free 3 end onto which the growing strand can be elongated DNA polymerase can t prime itself it must have a 3 end to get started These primers are actually RNA A special RNA polymerase (primase) puts them in Later the RNA primer is removed (by RNAse H in eukaryotes and DNA polymerase I in prokaryotes), and the gaps are filled in by DNA polymerase This may be a mechanism to enhance the fidelity of DNA replication DNA replication proceeds in both directions from the replication origin (bidirectional), which means you need to form two sets of replication complexes Each replication complex moves away from the origin (in opposite directions), unwinding and replicating both strands at each replication fork The strands of DNA are wound around each other like the strands of a rope As the strands are pulled apart during the movement of a replication fork, this unwinding tends to make the ends of the DNA turn (imagine unwinding the strands of a rope) Since the DNA is very long, twisted, and wrapped around histones, the DNA really can t turn its ends are rather tied down The unwinding of one region around the replication forks introduces strain into the regions of DNA that are still double-stranded, tending to make them wind tighter This is called supercoiling of the DNA Proteins (topoisomerases) are present to relieve the strain associated with helix unwinding by nicking and rejoining the DNA in the double-stranded regions At each replication fork there are two DNA polymerase complexes As the double-stranded DNA is unwound, two template strands are exposed One of the templates can be replicated in a continuous fashion by DNA polymerase since a continuous synthesis of new strands can occur in the 5 to 3 direction as the template strand is exposed Since all growing chains must be synthesized in the 5 to 3 direction, the lagging chain must be continuously reinitiated as new template is exposed The lagging strand is then synthesized discontinuously, in pieces that must be joined together later After synthesis, the RNA primers must be removed, gaps filled in, and the strands joined to give a linear, duplex DNA New histones are added to the lagging strand (which is now a duplex) while the old histones remain with the leading strand As the smoke slowly clears, we have a copy of the original DNA Since all DNA polymerases require a primer and work only in the 5 to 3 direction, there s a problem with replicating the 5 ends of the DNA If an RNA primer has to be laid down and later removed, these ends can t get replicated For bacteria with a circular genome, this isn t a problem Eukaryotes have specialized structures called telomeres at the.

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will set the value of the $sema semaphore back to 1 How you use the semaphore value is entirely up to you The usual method is to create a semaphore that relates to the available quantity of a specific resource

Because signals could interrupt the normal execution process of the script, particularly when working with threads, it can be a good idea to create a separate thread just for handling signals This is practical not only for multithreaded applications, but also for applications that make use of pipes, non-blocking I/O, and even networking Of course, by creating a new thread for signals, your script is now multithreaded, but it doesn t mean you have to create additional threads for handling the rest of your script To create a new signal-handling thread, all you do is import the Thread::Signal function:

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ends of the chromosome to solve this problem The exact details aren t known, but telomeres at the ends of each chromosome consist of a larger number (3000 to 12,000 base pairs) of a tandem (side by side) repeat of a G-rich sequence, (TTAGGG)n, in human DNA A specialized enzyme, telomerase, that also contains an RNA cofactor is responsible for replication of at least one strand of these telomeric sequences

use Thread::Signal;

15:

You also need to create an event handler for the Page Load event, so the page will open with the user s up-to-date profile information. Create that event the same way you did earlier, and then add the body of the method, highlighted in Example 9-6.

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This automatically generates a new thread and causes all signals to the program to be handled within this thread There is no difference in the way you set up signal handlers They can be assigned to existing functions or handled by anonymous subroutines, as usual The difference is that the signal handlers execute within the realm of a new thread This allows execution of the current process to continue when a signal is received There are some traps in using this technique Using die within a signal handler executed in the signal thread will cause the thread to exit, but won t necessarily cause the main thread to quit This also means you will have problems when using exit within an extension, since this too will affect the signal handler thread and not the main program

Polymerize in 5 -3 direction Proofread (check back for mistakes and remove them) exonuclease Remove RNA primer or remove damaged DNA exonuclease 3 5 5 3

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